tlr2 neutralizing antibody Search Results


anti-human tlr2 neutralizing antibodies  (ImmunoKontact)
90
ImmunoKontact anti-human tlr2 neutralizing antibodies
HSV and PAMPs induce IFN, ISGs, and proinflammatory cytokines in human primary macrophages. (A to C and G) Macrophages were infected with HSV-1 (17+, 5 × 105 PFU/ml, MOI of 1) or transfected with poly(I:C) [t-poly(I:C); 2 μg/ml] or poly(dA:dT) [t-poly(dA:dT); 2 μg/ml]. (A to C and G) After 6 h, after which RNA was harvested and mRNA accumulation of IFN-β, IFN-λ1, CXCL10, and TNF-α was assayed by real-time PCR. (D, E, and H) In similar experiments, cell supernatants were harvested after 20 h and assayed for the presence of TNF-α, CXCL10, and CCL3 by ELISA. (E, F, I, and K) Macrophages were infected with HSV or stimulated with IFN-α (100 IU/ml) or the agonist for <t>TLR2</t> (Pam3CSK4, 100 ng/ml), TLR3 [poly(I:C), 30 μg/ml], TLR7/8 (R848, 1 μg/ml), or TLR9 (ODN2216, 2 μg/ml). After 20 h of stimulation, supernatants were collected and protein levels assayed by ELISA. (J and L) Cells were infected with HSV-1 (17+ or KOS, 5 × 105 PFU/ml, MOI of 1) or HSV-2 (MOI of 1). Supernatants were harvested at 18 h and assayed by ELISA for CXCL10 and CCL3. The real-time PCR data are depicted as relative units (RU), which is a fold change in gene expression normalized to that of the endogenous reference gene 18S rRNA and is relative to the no template control (NTC) calibrator. The results depicted are means ± standard deviations (SD) within the shown experiment. Experiments were done five times (A and B) or two times (C to L), with similar findings.
Anti Human Tlr2 Neutralizing Antibodies, supplied by ImmunoKontact, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-human tlr2 neutralizing antibodies/product/ImmunoKontact
Average 90 stars, based on 1 article reviews
anti-human tlr2 neutralizing antibodies - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
anti-human tlr-2-neutralizing antibody t8050-15c  (Absolute Biotech Inc)
90
Absolute Biotech Inc anti-human tlr-2-neutralizing antibody t8050-15c
HSV and PAMPs induce IFN, ISGs, and proinflammatory cytokines in human primary macrophages. (A to C and G) Macrophages were infected with HSV-1 (17+, 5 × 105 PFU/ml, MOI of 1) or transfected with poly(I:C) [t-poly(I:C); 2 μg/ml] or poly(dA:dT) [t-poly(dA:dT); 2 μg/ml]. (A to C and G) After 6 h, after which RNA was harvested and mRNA accumulation of IFN-β, IFN-λ1, CXCL10, and TNF-α was assayed by real-time PCR. (D, E, and H) In similar experiments, cell supernatants were harvested after 20 h and assayed for the presence of TNF-α, CXCL10, and CCL3 by ELISA. (E, F, I, and K) Macrophages were infected with HSV or stimulated with IFN-α (100 IU/ml) or the agonist for <t>TLR2</t> (Pam3CSK4, 100 ng/ml), TLR3 [poly(I:C), 30 μg/ml], TLR7/8 (R848, 1 μg/ml), or TLR9 (ODN2216, 2 μg/ml). After 20 h of stimulation, supernatants were collected and protein levels assayed by ELISA. (J and L) Cells were infected with HSV-1 (17+ or KOS, 5 × 105 PFU/ml, MOI of 1) or HSV-2 (MOI of 1). Supernatants were harvested at 18 h and assayed by ELISA for CXCL10 and CCL3. The real-time PCR data are depicted as relative units (RU), which is a fold change in gene expression normalized to that of the endogenous reference gene 18S rRNA and is relative to the no template control (NTC) calibrator. The results depicted are means ± standard deviations (SD) within the shown experiment. Experiments were done five times (A and B) or two times (C to L), with similar findings.
Anti Human Tlr 2 Neutralizing Antibody T8050 15c, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-human tlr-2-neutralizing antibody t8050-15c/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
anti-human tlr-2-neutralizing antibody t8050-15c - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


HSV and PAMPs induce IFN, ISGs, and proinflammatory cytokines in human primary macrophages. (A to C and G) Macrophages were infected with HSV-1 (17+, 5 × 105 PFU/ml, MOI of 1) or transfected with poly(I:C) [t-poly(I:C); 2 μg/ml] or poly(dA:dT) [t-poly(dA:dT); 2 μg/ml]. (A to C and G) After 6 h, after which RNA was harvested and mRNA accumulation of IFN-β, IFN-λ1, CXCL10, and TNF-α was assayed by real-time PCR. (D, E, and H) In similar experiments, cell supernatants were harvested after 20 h and assayed for the presence of TNF-α, CXCL10, and CCL3 by ELISA. (E, F, I, and K) Macrophages were infected with HSV or stimulated with IFN-α (100 IU/ml) or the agonist for TLR2 (Pam3CSK4, 100 ng/ml), TLR3 [poly(I:C), 30 μg/ml], TLR7/8 (R848, 1 μg/ml), or TLR9 (ODN2216, 2 μg/ml). After 20 h of stimulation, supernatants were collected and protein levels assayed by ELISA. (J and L) Cells were infected with HSV-1 (17+ or KOS, 5 × 105 PFU/ml, MOI of 1) or HSV-2 (MOI of 1). Supernatants were harvested at 18 h and assayed by ELISA for CXCL10 and CCL3. The real-time PCR data are depicted as relative units (RU), which is a fold change in gene expression normalized to that of the endogenous reference gene 18S rRNA and is relative to the no template control (NTC) calibrator. The results depicted are means ± standard deviations (SD) within the shown experiment. Experiments were done five times (A and B) or two times (C to L), with similar findings.

Journal: Journal of Virology

Article Title: Early Innate Recognition of Herpes Simplex Virus in Human Primary Macrophages Is Mediated via the MDA5/MAVS-Dependent and MDA5/MAVS/RNA Polymerase III-Independent Pathways ▿

doi: 10.1128/JVI.01106-10

Figure Lengend Snippet: HSV and PAMPs induce IFN, ISGs, and proinflammatory cytokines in human primary macrophages. (A to C and G) Macrophages were infected with HSV-1 (17+, 5 × 105 PFU/ml, MOI of 1) or transfected with poly(I:C) [t-poly(I:C); 2 μg/ml] or poly(dA:dT) [t-poly(dA:dT); 2 μg/ml]. (A to C and G) After 6 h, after which RNA was harvested and mRNA accumulation of IFN-β, IFN-λ1, CXCL10, and TNF-α was assayed by real-time PCR. (D, E, and H) In similar experiments, cell supernatants were harvested after 20 h and assayed for the presence of TNF-α, CXCL10, and CCL3 by ELISA. (E, F, I, and K) Macrophages were infected with HSV or stimulated with IFN-α (100 IU/ml) or the agonist for TLR2 (Pam3CSK4, 100 ng/ml), TLR3 [poly(I:C), 30 μg/ml], TLR7/8 (R848, 1 μg/ml), or TLR9 (ODN2216, 2 μg/ml). After 20 h of stimulation, supernatants were collected and protein levels assayed by ELISA. (J and L) Cells were infected with HSV-1 (17+ or KOS, 5 × 105 PFU/ml, MOI of 1) or HSV-2 (MOI of 1). Supernatants were harvested at 18 h and assayed by ELISA for CXCL10 and CCL3. The real-time PCR data are depicted as relative units (RU), which is a fold change in gene expression normalized to that of the endogenous reference gene 18S rRNA and is relative to the no template control (NTC) calibrator. The results depicted are means ± standard deviations (SD) within the shown experiment. Experiments were done five times (A and B) or two times (C to L), with similar findings.

Article Snippet: Anti-human TLR2 neutralizing antibodies (ImmunoKontact and Imgenex) were added at a final concentration of 8.5 μg/ml 30 min prior to virus stimulation.

Techniques: Infection, Transfection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Expressing

HSV-1 infection activates cytokine expression independently of TLR2. Cells were infected with HSV-1 (17+, MOI of 1) or stimulated with TLR2 ligand Pam3CSK4 (100 ng/ml) in the presence or absence of TLR2 neutralizing antibodies (8.5 μg/ml). After 6 h, RNA was harvested, and after 18 h, supernatants were collected. Accumulation of TNF-α and IFN-β mRNA was assessed by real-time PCR (B and C), and the amounts of secreted TNF-α, CXCL10, and CCL3 were measured by ELISA (A, D, and E). RU, relative units. The results depicted are means ± SD from one of two experiments showing similar results.

Journal: Journal of Virology

Article Title: Early Innate Recognition of Herpes Simplex Virus in Human Primary Macrophages Is Mediated via the MDA5/MAVS-Dependent and MDA5/MAVS/RNA Polymerase III-Independent Pathways ▿

doi: 10.1128/JVI.01106-10

Figure Lengend Snippet: HSV-1 infection activates cytokine expression independently of TLR2. Cells were infected with HSV-1 (17+, MOI of 1) or stimulated with TLR2 ligand Pam3CSK4 (100 ng/ml) in the presence or absence of TLR2 neutralizing antibodies (8.5 μg/ml). After 6 h, RNA was harvested, and after 18 h, supernatants were collected. Accumulation of TNF-α and IFN-β mRNA was assessed by real-time PCR (B and C), and the amounts of secreted TNF-α, CXCL10, and CCL3 were measured by ELISA (A, D, and E). RU, relative units. The results depicted are means ± SD from one of two experiments showing similar results.

Article Snippet: Anti-human TLR2 neutralizing antibodies (ImmunoKontact and Imgenex) were added at a final concentration of 8.5 μg/ml 30 min prior to virus stimulation.

Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay